Promoter-sequence determinants and structural basis of primer-dependent transcription initiation in Escherichia coli.

ABSTRACT: Chemical modifications of RNA 5'-ends enable "epitranscriptomic" regulation, influencing multiple aspects of RNA fate. In transcription initiation, a large inventory of substrates compete with nucleoside triphosphates for use as initiating entities, providing an ab initio mechanism for altering the RNA 5'-end. In Escherichia coli cells, RNAs with a 5'-end hydroxyl are generated by use of dinucleotide RNAs as primers for transcription initiation, "primer-dependent initiation." Here, we use massively systematic transcript end readout (MASTER) to detect and quantify RNA 5'-ends generated by primer-dependent initiation for ∼4¹⁰ (∼1,000,000) promoter sequences in E. coli The results show primer-dependent initiation in E. coli involves any of the 16 possible dinuc

SUBMITTER: Skalenko KS 

PROVIDER: S-EPMC8271711 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature