Expression of an immunocomplex consisting of Fc fragment fused with a consensus dengue envelope domain III in Saccharomyces cerevisiae.

ABSTRACT:

Objectives

To explore Saccharomyces cerevisiae as an expression platform for dengue oral immune complex vaccine development.

Results

Molecular engineering was applied to create a fusion gene construct (scEDIII-PIGS) consisting of a yeast codon optimized sequence encoding for a synthetic consensus dengue envelope domain III (scEDIII) followed by a modified IgG Fc domain (PIGS). Northern blot showed transcription of the target gene, with a temporal expression pattern similar to those from previous work. Western blot showed assembly of various immune complexes from monomer to hexamer. Partial purification of scEDIII-PIGS was also attempted to demonstrate the feasibility of yeast system for immune complex vaccine development. Approximately 1 mg of scEDIII-PIGS can be produced fr

SUBMITTER: So KK 

PROVIDER: S-EPMC8272446 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature