Algae to angiosperms: Autofluorescence for rapid visualization of plant anatomy among diverse taxa.

ABSTRACT:

Premise

Fluorescence microscopy is an effective tool for viewing plant internal anatomy. However, using fluorescent antibodies or labels hinders throughput. We present a minimal protocol that takes advantage of inherent autofluorescence and aldehyde-induced fluorescence in plant cellular and subcellular structures to markedly increase throughput in cellular and ultrastructural visualization.

Methods and results

Twelve species distributed across the plant phylogeny were each subjected to five fixative treatments: 1% paraformaldehyde and 2% glutaraldehyde, 2% paraformaldehyde, 2% glutaraldehyde, formalin-acid-alcohol (FAA), and 70% ethanol. Samples were prepared by embedding and mechanically sectioning or via whole mount. A confocal laser scanning system was used to collect mi

SUBMITTER: Pegg TJ 

PROVIDER: S-EPMC8272585 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature