Detection of breast cancer stem cell gene mutations in circulating free DNA during the evolution of metastases.

ABSTRACT:

Purpose

Limited knowledge exists on the detection of breast cancer stem cell (BCSC)-related mutations in circulating free DNA (cfDNA) from patients with advanced cancers. Identification of new cancer biomarkers may allow for earlier detection of disease progression and treatment strategy modifications.

Methods

We conducted a prospective study to determine the feasibility and prognostic utility of droplet digital polymerase chain reaction (ddPCR)-based BCSC gene mutation analysis of cfDNA in patients with breast cancer.

Results

Detection of quantitative BCSC gene mutation in cfDNA by ddPCR mirrors disease progression and thus may represent a valuable and cost-effective measure of tumor burden. We have previously shown that hematological and neurological expressed 1-lik

SUBMITTER: Liu ZB 

PROVIDER: S-EPMC8272952 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature