The significance of SMARCB1 in the pathogenesis of renal cell carcinoma with rhabdoid features.

ABSTRACT:

Background

Renal cell carcinoma with rhabdoid features (RCC-RF) is an aggressive histologic variant in the adults and is usually unresponsive to standard chemotherapy.

Methods

Expression of SMARCB1/INI1 was examined in primary RCC-RF (n = 5). Stable INI1 with/without prostaglandin E2 receptor 1 (EP1) knockdown cell lines were created in the ACHN and 786-O RCC cell lines and measured for epidermal growth factor receptor (EGFR)-related signaling pathways. Chemosensitivity to targeted drugs in vitro was tested after knocking down of INI1 in both cell lines. The outcome of co-targeting of INI1 and EP1 in RCC was examined using a tumorigenicity assay.

Results

Expression of INI1 was markedly reduced at both transcriptional and translational levels in primary RCC-RF. Immunoh

SUBMITTER: Wang YW 

PROVIDER: S-EPMC8273225 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature