Loss of Claudin-3 Impairs Hepatic Metabolism, Biliary Barrier Function, and Cell Proliferation in the Murine Liver.

ABSTRACT:

Background & aims

Tight junctions in the liver are essential to maintain the blood-biliary barrier, however, the functional contribution of individual tight junction proteins to barrier and metabolic homeostasis remains largely unexplored. Here, we describe the cell type-specific expression of tight junction genes in the murine liver, and explore the regulation and functional importance of the transmembrane protein claudin-3 in liver metabolism, barrier function, and cell proliferation.

Methods

The cell type-specific expression of hepatic tight junction genes is described using our mouse liver single-cell sequencing data set. Differential gene expression in Cldn3^-/- and Cldn3^+/+ livers was assessed in young and aged mice by RNA sequencing (RNA-seq), and

SUBMITTER: Baier FA 

PROVIDER: S-EPMC8273426 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature