Peripheral autoreactive CD8 T-cell frequencies are too variable to be a reliable predictor of disease progression of human type 1 diabetes.

ABSTRACT:

Objectives

The detection of a peripheral immune cell signature that specifically reflects autoimmunity in type 1 diabetes would enable the prediction and staging of disease on an individual basis. However, defining such a signature is technically challenging. Reliable interpretation of immune cell-related biomarkers depends on their inherent variability and, to understand this variability, longitudinal analyses are required.

Methods

We performed a longitudinal observational study in which 40 individuals with elevated genetic risk of type 1 diabetes and persistent islet autoantibodies provided a blood sample every 4-6 weeks for > 1 year.

Results

Peripheral immune cell composition (T cells, NK cells and monocytes) was assessed using well-validated flow cytometry panels

SUBMITTER: Wesley JD 

PROVIDER: S-EPMC8273427 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature