Super-resolution fingerprinting detects chemical reactions and idiosyncrasies of single DNA pegboards.

ABSTRACT: We employ the single-particle fluorescence nanoscopy technique points accumulation for imaging in nanoscale topography (PAINT) using site-specific DNA probes to acquire two-dimensional density maps of specific features patterned on nanoscale DNA origami pegboards. We show that PAINT has a localization accuracy of ~10 nm that is sufficient to reliably distinguish dense (>10(4) features μm(-2)) sub-100 nm patterns of oligonucleotide features. We employ two-color PAINT to follow enzyme-catalyzed modification of features on individual origami and to show that single nanopegboards exhibit stable, spatially heterogeneous probe-binding patterns, or "fingerprints." Finally, we present experimental and modeling evidence suggesting that these fingerprints may arise from feature spacing variations th

SUBMITTER: Johnson-Buck A 

PROVIDER: S-EPMC8273705 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature