Identification and Characterization of a Master Transcription Factor of Th1 Cells, T-bet, Within Flounder (Paralichthys olivaceus).

ABSTRACT: T-bet, a T-box family member, is a transcription factor essential for the differentiation of naive CD4⁺ T cells into Th1 cells that are involved in both innate and adaptive immune responses. In this study, the transcription factor T-bet of flounder (Paralichthys olivaceus) was cloned and characterized, and its expression profile after infection was analyzed. T-bet⁺ cells were identified in flounder, and the expression and localization of T-bet in T lymphocyte subsets and B lymphocytes were investigated. Finally, the proliferation of T-bet⁺ cells, T lymphocyte subsets, and B lymphocytes were studied after stimulation with IFN-γ, IL-2, and IL-6, respectively, and the variations of some transcription factors and cytokines in CD4⁺ T lymphocyte

SUBMITTER: Tian H 

PROVIDER: S-EPMC8273736 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature