Complement C7 is Specifically Expressed in Mesangial Cells and is a Potential Diagnostic Biomarker for Diabetic Nephropathy and is Regulated by miR-494-3p and miR-574-5p.

ABSTRACT:

Background

Diabetic nephropathy (DN) is the leading cause of end-stage renal disease, but it remains relatively underdiagnosed.

Objective

In this study, we aimed to explore the key regulatory pathways and potential biomarkers related to DN using integrated bioinformatics analysis and validation.

Methods

First, the microarray data of the GSE30528 and GSE96804 datasets were downloaded from the Gene Expression Omnibus (GEO) database, and differentially expressed genes (DEGs) were screened. Then, weighted gene coexpression network analysis (WGCNA), gene ontology (GO) annotation, gene set enrichment analysis (GSEA) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to identify key pathways and genes. qRT-PCR and receiver operating characteri

SUBMITTER: Guo H 

PROVIDER: S-EPMC8273746 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature