A cassava common mosaic virus vector for virus-induced gene silencing in cassava.

ABSTRACT:

Background

Cassava is an important crop for food security and industry in the least-developed and developing countries. The completion of the cassava genome sequence and identification of large numbers of candidate genes by next-generation sequencing provide extensive resources for cassava molecular breeding and increase the need for rapid and efficient gene function analysis systems in cassava. Several plant virus-induced gene silencing (VIGS) systems have been developed as reverse genetic tools for rapid gene function analysis in cassava. However, these VIGS vectors could cause severe viral symptoms or inefficient gene silencing.

Results

In this study, we constructed agroinfection-compatible infectious cDNA clones of cassava common mosaic virus isolate CM (CsCMV-CM, genus

SUBMITTER: Tuo D 

PROVIDER: S-EPMC8273954 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature