Augmented peroxisomal ROS buffering capacity renders oxidative and thermal stress cross-tolerance in yeast.

ABSTRACT:

Background

Thermotolerant yeast has outstanding potential in industrial applications. Komagataella phaffii (Pichia pastoris) is a common cell factory for industrial production of heterologous proteins.

Results

Herein, we obtained a thermotolerant K. phaffii mutant G14 by mutagenesis and adaptive evolution. G14 exhibited oxidative and thermal stress cross-tolerance and high heterologous protein production efficiency. The reactive oxygen species (ROS) level and lipid peroxidation in G14 were reduced compared to the parent. Oxidative stress response (OSR) and heat shock response (HSR) are two major responses to thermal stress, but the activation of them was different in G14 and its parent. Compared with the parent, G14 acquired the better performance owing to its stronger OSR.

SUBMITTER: Lin NX 

PROVIDER: S-EPMC8273976 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature