Systematic identification of a panel of strong promoter regions from Listeria monocytogenes for fine-tuning gene expression.

ABSTRACT:

Background

Attenuated Listeria monocytogenes (Lm) has been widely used as a vaccine vector in the prevention and treatment of pathogen infection and tumor diseases. In addition, previous studies have proved that the attenuated Lm can protect zebrafish from Vibrio infections, indicating that the attenuated Lm has a good application prospect in the field of aquatic vaccines. However, the limitation mainly lies in the lack of a set of well-characterized natural promoters for the expression of target antigens in attenuated Lm.

Results

In our study, candidate strong promoters were identified through RNA-seq analysis, and characterized in Lm through enhanced green fluorescent protein (EGFP). Nine native promoters that showed stronger activities than that of the known strong promot

SUBMITTER: Ji Q 

PROVIDER: S-EPMC8273982 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature