A novel high-throughput screen for identifying lipids that stabilise membrane proteins in detergent based solution.

ABSTRACT: Membrane proteins have a range of crucial biological functions and are the target of about 60% of all prescribed drugs. For most studies, they need to be extracted out of the lipid-bilayer, e.g. by detergent solubilisation, leading to the loss of native lipids, which may disturb important protein-lipid/bilayer interactions and thus functional and structural integrity. Relipidation of membrane proteins has proven extremely successful for studying challenging targets, but the identification of suitable lipids can be expensive and laborious. Therefore, we developed a screen to aid the high-throughput identification of beneficial lipids. The screen covers a large lipid space and was designed to be suitable for a range of stability assessment methods. Here, we demonstrate its use as a tool for

SUBMITTER: Cecchetti C 

PROVIDER: S-EPMC8274869 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature