FisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria.

ABSTRACT: Little is known about mechanisms of membrane fission in bacteria despite their requirement for cytokinesis. The only known dedicated membrane fission machinery in bacteria, fission protein B (FisB), is expressed during sporulation in Bacillus subtilis and is required to release the developing spore into the mother cell cytoplasm. Here, we characterized the requirements for FisB-mediated membrane fission. FisB forms mobile clusters of approximately 12 molecules that give way to an immobile cluster at the engulfment pole containing approximately 40 proteins at the time of membrane fission. Analysis of FisB mutants revealed that binding to acidic lipids and homo-oligomerization are both critical for targeting FisB to the engulfment pole and membrane fission. Experiments using artificial membr

SUBMITTER: Landajuela A 

PROVIDER: S-EPMC8274934 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature