Metal-tagging Transmission Electron Microscopy for Localisation of Tombusvirus Replication Compartments in Yeast.

ABSTRACT: Positive-stranded (+) RNA viruses are intracellular pathogens in humans, animals and plants. To build viral replicase complexes (VRCs) viruses manipulate lipid flows and reorganize subcellular membranes. Redesigned membranes concentrate viral and host factors and create an environment that facilitates the formation of VRCs within replication organelles. Therefore, efficient virus replication depends on the assembly of specialized membranes where viral macromolecular complexes are turned on and hold a variety of functions. Detailed characterization of viral replication platforms in cells requires sophisticated imaging approaches. Here we present a protocol to visualize the three-dimensional organization of the tombusvirus replicase complex in yeast with MEtal-Tagging Transmission Electron M

SUBMITTER: De Castro IF 

PROVIDER: S-EPMC8275224 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature