Extraction of Small Molecules from Fecal Samples and Testingof Their Activity on Microbial Physiology.

ABSTRACT: The human body is colonized by vast communities of microbes, collectively known as microbiota, or microbiome. Although microbes colonize every surface of our bodies that is exposed to the external environment, the biggest collection of microbes colonizing humans and other mammals can be found in the gastrointestinal tract. Given the fact that the human gut is colonized by several hundred microbial species, our group hypothesized that the chemical diversity of this environment should be significant, and that many of the molecules present in that environment would have important signaling roles. Therefore, we devised a protocol to extract these molecules from human feces and test their signaling properties. Potentially bioactive extracts can be tested through addition to culture medium and a

SUBMITTER: Alves ES 

PROVIDER: S-EPMC8275228 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature