Resolving diverse protein-DNA footprints from exonuclease-based ChIP experiments.

ABSTRACT:

Motivation

High-throughput chromatin immunoprecipitation (ChIP) sequencing-based assays capture genomic regions associated with the profiled transcription factor (TF). ChIP-exo is a modified protocol, which uses lambda exonuclease to digest DNA close to the TF-DNA complex, in order to improve on the positional resolution of the TF-DNA contact. Because the digestion occurs in the 5'-3' orientation, the protocol produces directional footprints close to the complex, on both sides of the double stranded DNA. Like all ChIP-based methods, ChIP-exo reports a mixture of different regions associated with the TF: those bound directly to the TF as well as via intermediaries. However, the distribution of footprints are likely to be indicative of the complex forming at the DNA.

Results

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SUBMITTER: Biswas A 

PROVIDER: S-EPMC8275329 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature