Biomolecular solid-state NMR spectroscopy at 1200 MHz: the gain in resolution.

ABSTRACT: Progress in NMR in general and in biomolecular applications in particular is driven by increasing magnetic-field strengths leading to improved resolution and sensitivity of the NMR spectra. Recently, persistent superconducting magnets at a magnetic field strength (magnetic induction) of 28.2 T corresponding to 1200 MHz proton resonance frequency became commercially available. We present here a collection of high-field NMR spectra of a variety of proteins, including molecular machines, membrane proteins, viral capsids, fibrils and large molecular assemblies. We show this large panel in order to provide an overview over a range of representative systems under study, rather than a single best performing model system. We discuss both carbon-13 and proton-detected experiments, and show that in

SUBMITTER: Callon M 

PROVIDER: S-EPMC8275511 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature