Canine D₁₆₃-PrP polymorphic variant does not provide complete protection against prion infection in small ruminant PrP context.

ABSTRACT: E/D₁₆₃ polymorphism of dog prion protein (PrP) has been recently proposed as the variant responsible for canid prion resistance. To further investigate the protective role of this variant against prion replication, the transgenic mouse model OvPrP-Tg532 expressing sheep/goat PrP carrying the substitution D₁₆₂ (equivalent to D₁₆₃ position of dog PrP) was generated and intracranially inoculated with a broad collection of small ruminant prion strains. OvPrP-Tg532 mice showed resistance to classical bovine spongiform encephalopathy (BSE) from sheep and some classical scrapie isolates from sheep and goat but were susceptible to ovine atypical L-BSE and numerous classical scrapie isolates. Strikingly, some of these classical scrapie isolates showed a shift in the

SUBMITTER: Marin-Moreno A 

PROVIDER: S-EPMC8275588 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature