Ultra-fast scalable estimation of single-cell differentiation potency from scRNA-Seq data.

ABSTRACT:

Motivation

An important task in the analysis of single-cell RNA-Seq data is the estimation of differentiation potency, as this can help identify stem-or-multipotent cells in non-temporal studies or in tissues where differentiation hierarchies are not well established. A key challenge in the estimation of single-cell potency is the need for a fast and accurate algorithm, scalable to large scRNA-Seq studies profiling millions of cells.

Results

Here, we present a single-cell potency measure, called Correlation of Connectome and Transcriptome (CCAT), which can return accurate single-cell potency estimates of a million cells in minutes, a 100-fold improvement over current state-of-the-art methods. We benchmark CCAT against 8 other single-cell potency models and across 28 scRNA-Se

SUBMITTER: Teschendorff AE 

PROVIDER: S-EPMC8275983 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature