Next-generation humanized patient-derived xenograft mouse model for pre-clinical antibody studies in neuroblastoma.

ABSTRACT: Faithful tumor mouse models are fundamental research tools to advance the field of immuno-oncology (IO). This is particularly relevant in diseases with low incidence, as in the case of pediatric malignancies, that rely on pre-clinical therapeutic development. However, conventional syngeneic and genetically engineered mouse models fail to recapitulate the tumor heterogeneity and microenvironmental complexity of human pathology that are essential determinants of cancer-directed immunity. Here, we characterize a novel mouse model that supports human natural killer (NK) cell development and engraftment of neuroblastoma orthotopic patient-derived xenograft (O-PDX) for pre-clinical antibody and cytokine testing. Using cytotoxicity assays, single-cell RNA-sequencing, and multi-color flow cytometr

SUBMITTER: Nguyen R 

PROVIDER: S-EPMC8276267 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature