Transcription-coupled structural dynamics of topologically associating domains regulate replication origin efficiency.

ABSTRACT:

Background

Metazoan cells only utilize a small subset of the potential DNA replication origins to duplicate the whole genome in each cell cycle. Origin choice is linked to cell growth, differentiation, and replication stress. Although various genetic and epigenetic signatures have been linked to the replication efficiency of origins, there is no consensus on how the selection of origins is determined.

Results

We apply dual-color stochastic optical reconstruction microscopy (STORM) super-resolution imaging to map the spatial distribution of origins within individual topologically associating domains (TADs). We find that multiple replication origins initiate separately at the spatial boundary of a TAD at the beginning of the S phase. Intriguingly, while both high-efficiency an

SUBMITTER: Li Y 

PROVIDER: S-EPMC8276456 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature