Intratumor Heterogeneity of MIF Expression Correlates With Extramedullary Involvement of Multiple Myeloma.

ABSTRACT: Macrophage migration inhibitory factor (MIF) has been shown to promote disease progression in many malignancies, including multiple myeloma (MM). We previously reported that MIF regulates MM bone marrow homing and knockdown of MIF favors the extramedullary myeloma formation in mice. Here, based on MIF immunostaining of myeloma cells in paired intramedullary and extramedullary biopsies from 17 patients, we found lower MIF intensity in extramedullary MM (EMM) versus intramedullary MM (IMM). Flow cytometry and histology analysis in xenograft models showed a portion of inoculated human MM cells lost their MIF expression (MIF^Low) in vivo. Of note, IMM had dominantly MIF^High cells, while EMM showed a significantly increased ratio of MIF^Low cells. Furthermo

SUBMITTER: Xu J 

PROVIDER: S-EPMC8276700 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature