The Impact of Aging on Macroautophagy in the Pre-ovulatory Mouse Oocyte.

ABSTRACT: Accompanying the precipitous age-related decline in human female fertility is an increase in the proportion of poor-quality oocytes within the ovary. The macroautophagy pathway, an essential protein degradation mechanism responsible for maintaining cell health, has not yet been thoroughly investigated in this phenomenon. The aim of this study was to characterize the macroautophagy pathway in an established mouse model of oocyte aging using in-depth image analysis-based methods and to determine mechanisms that account for the observed changes. Three autophagy pathway markers were selected for assessment of gene and protein expression in this model: Beclin 1; an initiator of autophagosome formation, Microtubule-associated protein 1 light chain 3B; a constituent of the autophagosome membrane,

SUBMITTER: Peters AE 

PROVIDER: S-EPMC8277196 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature