Finite element analysis predicts Ca²⁺ microdomains within tubular-sarcoplasmic reticular junctions of amphibian skeletal muscle.

ABSTRACT: A finite element analysis modelled diffusional generation of steady-state Ca²⁺ microdomains within skeletal muscle transverse (T)-tubular-sarcoplasmic reticular (SR) junctions, sites of ryanodine receptor (RyR)-mediated SR Ca²⁺ release. It used established quantifications of sarcomere and T-SR anatomy (radial diameter [Formula: see text]; axial distance [Formula: see text]). Its boundary SR Ca²⁺ influx densities,[Formula: see text], reflected step impositions of influxes, [Formula: see text] deduced from previously measured Ca²⁺ signals following muscle fibre depolarization. Predicted steady-state T-SR junctional edge [Ca²⁺], [Ca²⁺]_edge, matched reported corresponding experimental cytosolic [Ca²⁺] eleva

SUBMITTER: Bardsley OJ 

PROVIDER: S-EPMC8277803 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature