Quantitative proton nuclear magnetic resonance method for simultaneous analysis of fluticasone propionate and azelastine hydrochloride in nasal spray formulation.

ABSTRACT: A facile, rapid, accurate and selective quantitative proton nuclear magnetic resonance (¹H-qNMR) method was developed for the simultaneous determination of fluticasone propionate (FLP) and azelastine hydrochloride (AZH) in pharmaceutical nasal spray for the first time. The ¹H-qNMR analysis of the studied analytes was performed using inositol as the internal standard and dimethyl sulfoxide-d₆ (DMSO-d₆) as the solvent. The quantitative selective proton signal of FLP was doublet of doublet at 6.290, 6.294, 6.316 and 6.319 ppm, while that of AZH was doublet at 8.292 and 8.310 ppm. The internal standard (inositol) produced a doublet signal at 3.70 and 3.71 ppm. The method was rectilinear over the concentration ranges of 0.25-20.0 and 0.2

SUBMITTER: El-Masry AA 

PROVIDER: S-EPMC8278066 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature