Bone marrow stromal cells (BMSCs CD45^- /CD44⁺ /CD73⁺ /CD90⁺ ) isolated from osteoporotic mice SAM/P6 as a novel model for osteoporosis investigation.

ABSTRACT: Available therapies aimed at treating age-related osteoporosis are still insufficient. Therefore, designing reliable in vitro model for the analysis of molecular mechanisms underlying senile osteoporosis is highly required. We have isolated and characterized progenitor cells isolated from bone marrow (BMSCs) of osteoporotic mice strain SAM/P6 (BMSC_SAM/P6 ). The cytophysiology of BMSC_SAM/P6 was for the first time compared with BMSCs isolated from healthy BALB/c mice (BMSC_BALB/c ). Characterization of the cells included evaluation of their multipotency, morphology and determination of specific phenotype. Viability of BMSCs cultures was determined in reference to apoptosis profile, metabolic activity, oxidative stress, mitochondrial membrane potential and casp

SUBMITTER: Sikora M 

PROVIDER: S-EPMC8278098 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature