Generation of Tetracycline and Rifamycin Resistant Chlamydia Suis Recombinants.

ABSTRACT: The Chlamydiaceae are a family of obligate intracellular, gram-negative bacteria known to readily exchange DNA by homologous recombination upon co-culture in vitro, allowing the transfer of antibiotic resistance residing on the chlamydial chromosome. Among all the obligate intracellular bacteria, only Chlamydia (C.) suis naturally integrated a tetracycline resistance gene into its chromosome. Therefore, in order to further investigate the readiness of Chlamydia to exchange DNA and especially antibiotic resistance, C. suis is an excellent model to advance existing co-culture protocols allowing the identification of factors crucial to promote homologous recombination in vitro. With this strategy, we co-cultured tetracycline-resistant wi

SUBMITTER: Marti H 

PROVIDER: S-EPMC8278220 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature