Defining T Cell Subsets in Human Tonsils Using ChipCytometry.

ABSTRACT: ChipCytometry is a multiplex imaging method that can be used to analyze either cell suspensions or tissue sections. Images are acquired by iterative cycles of immunostaining with fluorescently labeled Abs, followed by photobleaching, which allows the accumulation of multiple markers on a single sample. In this study, we explored the feasibility of using ChipCytometry to identify and phenotype cell subsets, including rare cell types, using a combination of tissue sections and single-cell suspensions. Using ChipCytometry of tissue sections, we successfully demonstrated the architecture of human palatine tonsils, including the B and T cell zones, and characterized subcompartments such as the B cell mantle and germinal center zone, as well as intrafollicular PD1-expressing CD4⁺ T ce

SUBMITTER: Hagel JP 

PROVIDER: S-EPMC8278278 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature