Refinement of the spinal cord injury rat model and validation of its applicability as a model for memory loss and chronic pain.

ABSTRACT:

Background

Laminectomy produces trauma in spinal cord injury (SCI) animal models resulting in impinging artefacts and welfare issues. Mechanizing laminectomy using a dental burr assisted (DBA) technique to reduce the impact of conventionally performed laminectomy on animal welfare without any alterations in the outcome of the model was previously demonstrated. However, further validation was necessary to establish it as an alternative in developing SCI rats as a model of chronic pain and memory loss.

Novel method

DBA technique was employed to perform laminectomy at T10-T11 vertebrae in rats undergoing contusion SCI as a model of chronic pain and memory loss. In a 56-day study, 24 female Wistar rats (Crl: WI) were assigned randomly to four equal groups: conventionally laminec

SUBMITTER: Harikrishnan VS 

PROVIDER: S-EPMC8278431 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature