Nelfinavir restricts A549 cell growth by inhibiting STAT3 signaling.

ABSTRACT:

Objective

To investigate the anticancer effect of nelfinavir (NFV) on human A549 cells.

Methods

The inhibitory effects of NFV on the proliferation of human A549 cells were assessed using a MTT assay. Apoptotic cells were observed by fluorescence microscopy following Hoechst 33342 staining. Apoptosis of A549 cells was assessed using Annexin-V/propidium iodide staining and flow cytometry. Expression levels of signal transducer and activator of transcription 3 (STAT3) and p-STAT3 were measured by western blotting. STAT3 RNA silencing was used to investigate the pro-apoptotic mechanism of NFV in A549 cells.

Results

NFV dose-dependently suppressed proliferation of human A549 cells and induced significant apoptosis. Western blotting showed that the antitumor function of NFV

SUBMITTER: He F 

PROVIDER: S-EPMC8278455 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature