Identification of Novel Mast Cell Activators Using Cell-Based High-Throughput Screening.

ABSTRACT: Mast cells (MCs) are known to regulate innate and adaptive immunity. MC activators have recently been described as safe and effective vaccine adjuvants. Many currently known MC activators are inadequate for in vivo applications, however, and research on identifying novel MC activators is limited. In this study, we identified novel MC activators by using high-throughput screening (HTS) assays using approximately 55,000 small molecules. Data sets obtained by the primary HTS assays were statistically evaluated using quality control rules and the B-score calculation, and compounds with B-scores of >3.0 were chosen as mast cell activators (hits). These hits were re-evaluated with secondary and tertiary HTS assays, followed by further statistical analysis. From these hits, we selected 15 compoun

SUBMITTER: Choi HW 

PROVIDER: S-EPMC8278568 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature