Expression of antibody fragments in Saccharomyces cerevisiae strains evolved for enhanced protein secretion.

ABSTRACT: Monoclonal antibodies, antibody fragments and fusion proteins derived thereof have revolutionized the practice of medicine. Major challenges faced by the biopharmaceutical industry are however high production costs, long processing times and low productivities associated with their production in mammalian cell lines. The yeast Saccharomyces cerevisiae, a well-characterized eukaryotic cell factory possessing the capacity of post-translational modifications, has been industrially exploited as a secretion host for production of a range of products, including pharmaceuticals. However, due to the incompatible surface glycosylation, few antibody molecules have been functionally expressed in S. cerevisiae. Here, three non-glycosylated antibody fragments from human and the Camelidae family were ch

SUBMITTER: Wang Y 

PROVIDER: S-EPMC8278646 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature