TRNA derived fragment (tRF)-3009 participates in modulation of IFN-α-induced CD4⁺ T cell oxidative phosphorylation in lupus patients.

ABSTRACT:

Background

Accumulating evidence suggests tRNA-derived fragments (tRFs) play important roles in cellular homeostasis. Here we aimed to explore aberrant expression of tRFs in CD4⁺ T cells from patients with systemic lupus erythematosus (SLE) and their potential function in the SLE pathogenesis.

Methods

First, small RNA sequencing was performed on CD4⁺ T cells from four SLE patients and three healthy controls (HCs). Candidate tRFs were then validated in CD4⁺ T cells from 97 SLE patients and their relevant disease controls using qRT-PCR. Then sequencing was used to investigate the profiles of HC-derived CD4⁺ T cells transfected with tRF-3009. Lastly, tRF-3009 siRNA or tRF-3009 mimics were transfected into CD4⁺ T cells with/

SUBMITTER: Geng G 

PROVIDER: S-EPMC8278670 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature