Influence of the mesenchymal stromal cell source on the hematopoietic supportive capacity of umbilical cord blood-derived CD34⁺-enriched cells.

ABSTRACT:

Background

Umbilical cord blood (UCB) is a clinically relevant alternative source of hematopoietic stem/progenitor cells (HSPC). To overcome the low cell number per UCB unit, ex vivo expansion of UCB HSPC in co-culture with mesenchymal stromal cells (MSC) has been established. Bone marrow (BM)-derived MSC have been the standard choice, but the use of MSC from alternative sources, less invasive and discardable, could ease clinical translation of an expanded CD34⁺ cell product. Here, we compare the capacity of BM-, umbilical cord matrix (UCM)-, and adipose tissue (AT)-derived MSC, expanded with/without xenogeneic components, to expand/maintain UCB CD34⁺-enriched cells ex vivo.

Methods

UCB CD34⁺-enriched cells were isolated from cryopreserved mo

SUBMITTER: Bucar S 

PROVIDER: S-EPMC8278708 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature