Implementation of an open-source robotic platform for SARS-CoV-2 testing by real-time RT-PCR.

ABSTRACT: The current global pandemic due to the SARS-CoV-2 has pushed the limits of global health systems across all aspects of clinical care, including laboratory diagnostics. Supply chain disruptions and rapidly-shifting markets have resulted in flash-scarcity of commercial laboratory reagents; this has motivated health care providers to search for alternative workflows to cope with the international increase in demand for SARS-CoV-2 testing. The aim of this study is to present a reproducible workflow for real time RT-PCR SARS-CoV-2 testing using OT-2 open-source liquid-handling robots (Opentrons, NY). We have developed a framework that includes a code template which is helpful for building different stand-alone robotic stations, capable of performing specific protocols. Such stations can be comb

SUBMITTER: Villanueva-Canas JL 

PROVIDER: S-EPMC8279358 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature