Cardiac Glycoside Ouabain Exerts Anticancer Activity via Downregulation of STAT3.

ABSTRACT: Cardiac glycosides are plant-derived steroid-like compounds which have been used for the treatment of cardiovascular diseases. Ouabain, a cardiotonic steroid and specific Na⁺/K⁺-ATPase inhibitor, has been rediscovered for its potential use in the treatment of cancer. However, the cellular targets and anticancer mechanism of ouabain in various cancers remain largely unexplored. In this study, we confirmed the cytotoxic effects of ouabain on several cancer cell lines. Further examination revealed the increase of apoptosis, intracellular ROS generation and DNA double-strand breaks induced by ouabain treatment. Besides, ouabain effectively suppressed STAT3 expression as well as phosphorylation in addition to block STAT3-mediated transcription and downstream target protein

SUBMITTER: Du J 

PROVIDER: S-EPMC8279743 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature