Tobacco BY2 cells expressing recombinant cardosin B as an alternative for production of active milk clotting enzymes.

ABSTRACT: Cynara cardunculus L. or cardoon is a plant that is used as a source of milk clotting enzymes during traditional cheese manufacturing. This clotting activity is due to aspartic proteases (APs) found in the cardoon flower, named cyprosins and cardosins. APs from cardoon flowers display a great degree of heterogeneity, resulting in variable milk clotting activities and directly influencing the final product. Producing these APs using alternative platforms such as bacteria or yeast has proven challenging, which is hampering their implementation on an industrial scale. We have developed tobacco BY2 cell lines as an alternative plant-based platform for the production of cardosin B. These cultures successfully produced active cardosin B and a purification pipeline was developed to obtain isolate

SUBMITTER: Folgado A 

PROVIDER: S-EPMC8280186 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature