Increased Retention of Cardiac Cells to a Glass Substrate through Streptavidin-Biotin Affinity.

ABSTRACT: In vitro analysis of primary isolated adult cardiomyocyte physiological processes often involves optical imaging of dye-loaded cells on a glass substrate. However, when exposed to rapid solution changes, primary cardiomyocytes often move to compromise quantitative measures. Improved immobilization of cells to glass would permit higher throughput assays. Here, we engineer the peripheral membrane of cardiomyocytes with biotin to anchor cardiomyocytes to borosilicate glass coverslips functionalized with streptavidin. We use a rat cardiac myoblast cell line to determine general relationships between processing conditions, ligand density on the cell and the glass substrate, cellular function, and cell retention under shear flow. Use of the streptavidin-biotin system allows for more than 80% ret

SUBMITTER: Davis KA 

PROVIDER: S-EPMC8280672 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature