Probing the Conformational States of a pH-Sensitive DNA Origami Zipper via Label-Free Electrochemical Methods.

ABSTRACT: DNA origami structures represent an exciting class of materials for use in a wide range of biotechnological applications. This study reports the design, production, and characterization of a DNA origami "zipper" structure, which contains nine pH-responsive DNA locks. Each lock consists of two parts that are attached to the zipper's opposite arms: a DNA hairpin and a single-stranded DNA that are able to form a DNA triplex through Hoogsteen base pairing. The sequences of the locks were selected in a way that the zipper adopted a closed configuration at pH 6.5 and an open state at pH 8.0 (transition pK_a 7.6). By adding thiol groups, it was possible to immobilize the zipper structure onto gold surfaces. The immobilization process was characterized electrochemically to confirm

SUBMITTER: Williamson P 

PROVIDER: S-EPMC8280702 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature