Imidazolium labelling permits the sensitive mass-spectrometric detection of N-glycosides directly from serum.
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ABSTRACT:
SUBMITTER: Zhang YY
PROVIDER: S-EPMC8280963 | biostudies-literature | 2021 Jul
REPOSITORIES: biostudies-literature
Publications
Imidazolium labelling permits the sensitive mass-spectrometric detection of <i>N</i>-glycosides directly from serum.
Zhang Yao-Yao YY Ghirardello Mattia M Wang Ting T Lu Ai-Min AM Liu Li L Voglmeir Josef J Galan M Carmen MC
Chemical communications (Cambridge, England) 20210701 57
A novel imidazolium derivative (GITag) shows superior ionisation and consequently allows increased mass spectrometric detection capabilities of oligosaccharides and N-glycans. Here we demonstrate that human serum samples can be directly labelled by GITag on a MALDI target plate, abrogating prevalently required sample pretreatment or clean-up steps. ...[more]
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