Senescent cells exacerbate chronic inflammation and contribute to periodontal disease progression in old mice.

ABSTRACT:

Background

Coinciding with other chronic comorbidities, the prevalence of periodontal disease increases with aging. Mounting evidence has established that senescent cells accumulate at sites of age-related pathologies, where they promote "non-microbial" inflammation. We hypothesized that alveolar bone osteocytes develop senescence characteristics in old age.

Methods

Alveolar bone samples were obtained from young (6 months) and old (20 to 22 months) mice to evaluate the expression of senescence biomarkers by immunofluorescent staining. Osteocyte-enriched fractions were used to characterize the age-related senescence-associated secretory phenotype (SASP) gene expression profile. Primary alveolar bone cells were exposed to the SASP via in vitro senescent conditioned media (SCM)

SUBMITTER: Aquino-Martinez R 

PROVIDER: S-EPMC8281492 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature