The effect of IL-2 stimulation and treatment of TRPM3 on channel co-localisation with PIP₂ and NK cell function in myalgic encephalomyelitis/chronic fatigue syndrome patients.

ABSTRACT:

Background

Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a serious multifactorial disorder. The origin remains ambiguous, however reduced natural killer (NK) cell cytotoxicity is a consistent immunological feature of ME/CFS. Impaired transient receptor potential melastatin 3 (TRPM3), a phosphatidylinositol dependent channel, and impaired calcium mobilisation have been implicated in ME/CFS pathology. This investigation aimed to examine the localisation of TRPM3 at the NK cell plasma membrane and co-localisation with phosphatidylinositol 4,5-bisphosphate (PIP₂). The effect of IL-2 priming and treatment using pregnenolone sulfate (PregS) and ononetin on TRPM3 co-localisation and NK cell cytotoxicity in ME/CFS patients and healthy controls (HC) was also inve

SUBMITTER: Eaton-Fitch N 

PROVIDER: S-EPMC8281618 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature