Viability, yield and expansion capability of feline MSCs obtained from subcutaneous and reproductive organ adipose depots.

ABSTRACT:

Background

The source of multipotent stromal cells (MSC) can have a significant influence on the health and expansion capacity of the cells. As the applications for allogeneic MSCs in the treatment of feline diseases increase, the location of the initial donor tissue must be analyzed. To date, comparisons have only been made between feline MSCs collected from bone marrow or abdominal fat. This is the first report to compare cells obtained from different adipose depots in the cat with a focus on clinically relevant donor tissues. The tissue was collected from 34 healthy cats undergoing spaying (fat around the ovaries and uterine horn) or subcutaneous fat collected during surgical procedures.

Results

The amount of starting material is essential to isolate sufficient MSCs. The

SUBMITTER: Wysong A 

PROVIDER: S-EPMC8281647 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature