Biochemical and phenotypic characterisation of the Mycobacterium smegmatis transporter UspABC.

ABSTRACT: Mycobacterium tuberculosis (Mtb) is an intracellular human pathogen that has evolved to survive in a nutrient limited environment within the host for decades. Accordingly, Mtb has developed strategies to acquire scarce nutrients and the mycobacterial transporter systems provide an important route for the import of key energy sources. However, the physiological role of the Mtb transporters and their substrate preference(s) are poorly characterised. Previous studies have established that the Mtb UspC solute-binding domain recognises amino- and phosphorylated-sugars, indicating that the mycobacterial UspABC transporter plays a key role in the import of peptidoglycan precursors. Herein, we have used a wide array of approaches to investigate the role of UspABC

SUBMITTER: Karlikowska M 

PROVIDER: S-EPMC8281650 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature