Infectious titer determination of lentiviral vectors using a temporal immunological real-time imaging approach.

ABSTRACT: The analysis of the infectious titer of the lentiviral vector samples obtained during upstream and downstream processing is of major importance, however, also the most challenging method to be performed. Currently established methods like flow cytometry or qPCR lack the capability of enabling high throughput sample processing while they require a lot of manual handling. To address this limitation, we developed an immunological real-time imaging method to quantify the infectious titer of anti-CD19 CAR lentiviral vectors with a temporal readout using the Incucyte® S3 live-cell analysis system. The infective titers determined with the Incucyte® approach when compared with the flow cytometry-based assay had a lower standard deviation between replicates and a broader linear range. A major advan

SUBMITTER: Labisch JJ 

PROVIDER: S-EPMC8281989 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature