Aqueous two-phase deposition and fibrinolysis of fibroblast-laden fibrin micro-scaffolds.

ABSTRACT: This paper describes printing of microscale fibroblast-laden matrices using an aqueous two-phase approach that controls thrombin-mediated enzymatic crosslinking of fibrin. Optimization of aqueous two-phase formulations enabled polymerization of consistent sub-microliter volumes of cell-laden fibrin. When plasminogen was added to these micro-scaffolds, the primary normal human lung fibroblasts converted it to plasmin, triggering gradual degradation of the fibrin. Time-lapse live-cell imaging and automated image analysis provided readouts of time to degradation of 50% of the scaffold as well as maximum degradation rate. The time required for degradation decreased linearly with cell number while it increased in a dose-dependent manner upon addition of TGF-β1. Fibroblasts isolated from idiopat

SUBMITTER: Robinson S 

PROVIDER: S-EPMC8282251 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature