Mitochondrial DNA Damage and Brain Aging in Human Immunodeficiency Virus.

ABSTRACT:

Background

Neurocognitive impairment (NCI) remains common in people living with human immunodeficiency virus (PLWH), despite suppressive antiretroviral therapy (ART), but the reasons remain incompletely understood. Mitochondrial dysfunction is a hallmark of aging and of neurodegenerative diseases. We hypothesized that human immunodeficiency virus (HIV) or ART may lead to mitochondrial abnormalities in the brain, thus contributing to NCI.

Methods

We studied postmortem frozen brain samples from 52 PLWH and 40 HIV-negative controls. Cellular mitochondrial DNA (mtDNA) content and levels of large-scale mtDNA deletions were measured by real-time polymerase chain reaction. Heteroplasmic mtDNA point mutations were quantified by deep sequencing (Illumina). Neurocognitive data were ta

SUBMITTER: Roca-Bayerri C 

PROVIDER: S-EPMC8282328 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature