Functional interdependence of the actin nucleator Cobl and Cobl-like in dendritic arbor development.

ABSTRACT: Local actin filament formation is indispensable for development of the dendritic arbor of neurons. We show that, surprisingly, the action of single actin filament-promoting factors was insufficient for powering dendritogenesis. Instead, this required the actin nucleator Cobl and its only evolutionary distant ancestor Cobl-like acting interdependently. This coordination between Cobl-like and Cobl was achieved by physical linkage by syndapins. Syndapin I formed nanodomains at convex plasma membrane areas at the base of protrusive structures and interacted with three motifs in Cobl-like, one of which was Ca²⁺/calmodulin-regulated. Consistently, syndapin I, Cobl-like's newly identified N terminal calmodulin-binding site and the single Ca²⁺/calmodulin-responsive syndapin-b

SUBMITTER: Izadi M 

PROVIDER: S-EPMC8282341 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature